BACILLUS
Bacillus
represents a genus of Gram-positive bacteria which are ubiquitous in nature
(soil, water, and airborne dust). Some species are natural flora in the human
intestines. When grown on blood agar, Bacillus produces large, spreading,
gray-white colonies with irregular margins. A unique characteristic of this
bacterium is its ability to produce endospores when environmental
conditions are stressful. The only other known spore-producing bacterium is
Clostridium(anaerobic). Although most species of Bacillus are
harmless saprophytes, two species are considered medically significant:
B.anthracis and B. cereus.
(under the microscope)
B. anthracis
B. anthracis is the bacterium which causes
anthrax in cows, sheep, and sometimes humans. Anthrax is transmitted to
humans via direct contact with animal products or inhalation of endospores.
Under the microscope, B. anthracis cells appear to have square ends and
seem to be attached by a joint to other cells. The spores are best observed when
the bacterium is cultured on artificial media. Sources of infection are usually
industrial or agricultural and the infection is classified as one of three
types:
- CUTANEOUS INFECTION (95% of human cases)
- INHALATION ANTHRAX (rare)
- GASTROINTESTINAL ANTHRAX (very rare!)
Anthrax can be treated with
penicillin or tetracycline. A vaccine is available but is only used on
individuals at high risk of contraction.
LABORATORY INDICATIONS:
- Nonhemolytic (sheep blood agar)
- Non-motile
- Gel hydrolysis -
- Catalase +
B. cereus
Unlike B. anthracis, B.cereus is a motile
bacterium which can cause toxin-mediated food poisoning. It is known to inhabit
many kinds of food including stew, cereal, and milk. Most recently, however, it
has been found in fried rice. The two toxins released by the bacterium lead to
vomiting and diarrhea, symptoms similar to those of Staphylococcus food
poisoning. Because toxin production usually takes place after the infected foods
are cooked, proper cold storage of food is recommended immediately after
preparation.
LABORATORY INDICATIONS:
- Hemolytic (sheep blood agar)
- Motile
- Gel hydrolysis +
- Glucose, maltose, & salicin fermentative
- Catalase +
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