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HELICOBACTER PYLORI AND MUCOSAL INFLAMMATION :
clinical consequences in the adult.


The gastric mucosa is equipped with specific physiological functions and can trigger off a range of inflammatory and immune reactions according to aggression mechanism. The term gastritis designates any inflammatory lesion of the gastric mucosa in response to an aggression of the stomach. The discovery of Helicobacter pylori (H. pylori) in 1982 showed it to be responsible for the most frequent forms of chronic gastritis and led to the histopathological classification of gastritis being re-organised (Sydney system, etc.).

CHRONIC H. PYLORI GASTRITIS
H. pylori is a flagellate bacterium whose great mobility allows it to cross the thick layer of mucus and reach the gastric epithelium. Here, it can attach itself and propagate, while protecting itself from acidity by alkalinising the ambient milieu through its urease activity. H. pylori infection results in an acute, then chronic, inflammation of the gastric mucosa, as shown by experimental infections of the human volunteer, and in the animal. The inflammation regresses following antimicrobial treatment.

H. pylori can break down the protective mucus layer and damage the gastric epithelium by various means, primarily toxins. Deterioration of intercellular junctions allows it to penetrate between the epithelial cells. The first host response to infection is acute inflammation resulting from the arrival of numerous polymorphonuclear cells (PMNs) attracted by the chemotactic factors produced by the bacteria and the aggressed mucosa (cytokines by the epithelial cells and inflammatory cells). The PMNs migrate to the surface of the mucosa and phagocytise the bacteria. In doing so, they release toxic substances which contribute towards maintaining the lesions in the mucosa. If the infection is not eliminated, the second phase comes into play, characterised by an active, chronic inflammation and a specific mucosal immune response with the large-scale production of cytokines (interleukins 6 and 8, TNF alpha, etc.). The PMNs are less numerous (their persistence is a sign of the gastritic activity) and the inflammatory infiltrate is essentially made up of mononuclear cells: macrophages, B and T lymphocytes, and plasma cells producing specific immunoglobulins (secretory IgA, etc.). One of the characteristics of H. pylori infection is the growth of lymphoid follicles, indicating the gastric mucosa's possible acquisition of an organised lymphoid tissue named MALT (mucosa-associated lymphoid tissue). Crossed immune reactions between H. pylori and host antigens (homologous "stress" proteins, antibacterial antibodies directed against the mucosa components) could become involved in the lesions' pathogenesis. H. pylori strains producing cytotoxins and/or expressing particular markers (type I strains expressing the CagA protein, etc.) are found more commonly in cases of peptic ulceration and atrophic gastritis. They are associated with an increase of the inflammatory reaction (recruitment and activation of the PMNs) and of the immune response (epithelial cell production of interleukin 8), probably responsible for a greater disease severity. The influence of the host is also determinant in expression of the infection, particularly as concerns control of the immune response (negative regulation to attenuate the lesional consequences) and disturbances to the gastric physiology associated with H. pylori infection ...

Although H. pylori is always associated with an active chronic inflammatory reaction, deteriorations to the epithelial surface, degree of spread of inflammation (antral gastritis or pangastritis), development of atrophy and intestinal metaplasia seem to be variable aspects of response to the infection.
Their appearance seems to depend on factors proper to the bacterium ("ulcerogenic" or "cytotoxic" strains) and/or the host (inflammatory and immune responses, extent and functional condition of the acid-secreting fundal mucosa), plus contributions from endogenous (action of the bile, etc.) and exogenous factors (role of diet, etc.) ...

H. PYLORI GASTRITIS AND ASSOCIATED PATHOLOGIES

Chronic H. pylori-induced gastritis is generally asymptomatic and without major lesional consequence (superficial chronic gastritis). Some subjects, however, do develop multifactorial gastroduodenal diseases along with the infectious gastritis: duodenal ulcer, gastric ulcer, primitive gastric lymphoma, gastric adenocarcinoma. These fundamental differences in expression seem to be related to the host-bacterium interactions associated with environmental factors.

GASTRIC OR DUODENAL PEPTIC ULCER

Excluding those caused by non-steroidal anti-inflammatory drugs (NSAIDs), duodenal and gastric ulcers are both closely associated with H. pylori infection. Eradicating H. pylori promotes healing of duodenal ulcers and eliminates virtually all gastric or duodenal ulcer relapse, i.e. the ulcer is cured. H. pylori - gastritis possibly plays a facilitating role in ulcers resulting from NSAIDs, but this remains a matter of debate.

The mode of action of H. pylori infection has been studied most in duodenal ulcerogenesis. H. pylori is often found in the duodenal mucosa of ulcer patients, solely at the gastric metaplasia which develops there because of the hyperacidity. Colonisation of the duodenal mucosa, generally at the cap, is said to be the cause of the active chronic duodenitis which persists between ulcerous attacks. The relative risk of developing a duodenal ulcer is very high (51 times) in the event of H. pylori being present in the duodenum. Chronic H. pylori infection generally elicits hyperacid secretion (reversible after the bacterium has been eradicated) which is even more so in patients with duodenal ulcer. Clinical and experimental data suggest a chain of phenomena. The bacterium are thought to decrease antral D-cell production of somatostatin, thus suppressing the physiological brake to gastrin secretion by antral G-cells. The resulting hypergastrinaemia (perhaps also elicited by the gastric inflammatory reaction) would stimulate the fundal parietal cells, causing the proton pumps to increase H+ ion release and, in ulcer patients perhaps, the parietal cell mass to increase. The acid hypersecretion would result in an increase of duodenal acid load, predisposing ulcers to occur at the duodenal mucosa weakened by inflammation.

GASTRIC NEOPLASIAS

Given the almost constant association of mucosal infection with these tumoral proliferations and their regression following eradication of the bacterium, H. pylori is probably involved in MALT gastric lymphomas. T lymphocyte stimulation by bacterial antigens seems to be an essential mechanism of lymphocyte proliferation in MALT gastric lymphomas, which probably develop from the lymphoid follicle reaction typical of H. pylori infection.

A statistical association between H. pylori infection and gastric adenocarcinoma (intestinal or diffuse) has been demonstrated. The active chronic gastritis induced by H. pylori seems the starting point of carcinogenesis.
H. pylori is also implicated in the atrophying development of chronic gastritis, the necessary condition for the emergence of intestinal metaplasia and dysplasia lesions, the stages preceding the development of gastric cancer under the influence of mutagenic factors. H. pylori gastritis could also be involved in decreasing the gastric lumen vitamin C concentration and in increasing epithelial cell proliferation, factors acknowledged to be of influence in carcinogenesis. For these reasons, in 1994 the WHO decided to put H. pylori in stomach carcinogen class 1.

DYSPEPSIA NON-ULCER

H. pylori's responsibility in functional or non-ulcer dyspepsia (NUD), a rather mixed and elusive bag, has not been demonstrated. H. pylori infection seems commoner in patients with NUD than in the population at large. Nevertheless, the various therapeutic studies published on the subject showed conflicting results. Whereas some highlight particular symptomatic profiles, such as ulcer-like pain, others do not. Be this as it may, the improvement of symptoms after the treatment, observed only after the bacterium has been eradicated, indicates the role of H. pylori in non-ulcer patients presenting epigastralgias. Rigorous methodological research is the solution.

CONCLUSION

The discovery of H. pylori triggered off a revival in interest for gastric inflammatory pathology. This led to an overhaul in the classification of chronic gastritis and a better understanding of the gastric mucosal barrier and its means of defence, in which mucus occupies a key place. Mucosal infection by H. pylori results in active chronic inflammation and lymphoid follicle reaction. It is the common denominator of frequent and multifactorial gastroduodenal diseases (duodenal ulcer, gastric ulcer, MALT gastric lymphoma, gastric adenocarcinoma, etc.). Host-H. pylori interactions and their consequences on gastric physiological functions (acid secretion, etc.) and environmental factors together determine expression of the disease.


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